Abstract： A high-performance liquid chromatography method has been established for the simultaneous determination of urea and biuret content in diesel vehicle exhaust treatment solution. The pretreatment technology of mobile phase dilution and direct membrane passing was adopted，methanol: potassium dihydrogen phosphate aqueous solution (0.074mol /L) =5:95 was used as the mobile phase for elution, and the samples were separated by C18 analytical column, and the content of the samples was detected by DAD detector.The experimental resulted show that urea and biuret had a good linear relationship within a series of concentration ranges, the correlation coefficients R2 were 0.999 4 and 0.999 6, the detection limits were 0.003 5 and 0.0002 mg/mL, the recovery rates were 100.1%~110.4%, and the relative standard deviation RSD were 0.4%~1.4%.This method is simple in sample pretreatment, simple and fast in analysis, and can be used for the detection and analysis of urea and biuret content in diesel vehicle exhaust treatment solution.
杨照生，耿晓红，戴丽明，罗 强，郑彦宁，蔡立鹏，李 涛，张京京. 柴油车尾气处理液中尿素与缩二脲含量的测定[J]. 煤炭与化工, 2023, 46(6): 143-147.
Yang Zhaosheng, Geng Xiaohong, Dai Liming, Luo Qiang, Zheng Yanning, Cai Lipeng, Li Tao, Zhang Jingjing. Determination of urea and biuret content in diesel vehicle exhaust treatment fluid. CCI, 2023, 46(6): 143-147.
[ 1 ] 朱宗敏，杨晏泉，孙 杰. 柴油车尾气处理液应用展望[ J ]. 化工环保，2020，40（ 6 ）：679 - 683.
[ 2 ] 张洪涛. 国六排放车用柴油机SCR系统的研究与开发[ D ]. 天津：天津大学，2019.
[ 3 ] 王 攀，李红明，陈鹏姣，等. 车用尿素溶液杂质问题研究总结[ J ]. 山东化工，2020，49（ 17 ）：128 - 129.
[ 4 ] 王志江. 车用尿素溶液及其应用[ J ]. 化工管理，2020（ 32 ）：101 - 102.
[ 5 ] 郝春晓，陈伟程，谢 琼，等. 车用尿素溶液质量对SCR系统的影响[ J ]. 环境与可持续发展，2016，41（ 1 ）：46 - 49.
[ 6 ] Jeong Byeong Gyu, Oh Kwang Chul, Jang Seong Uk.Melting and Heat Transfer Characteristics of Urea Water Solution According to a Heating Module’s Operating Conditions in a Frozen Urea Tank[ J ]. Energies, 2021, 14（ 23 ）：8 164.
[ 7 ] GB 29518-2013, 柴油发动机氮氧化物还原剂尿素水溶液（AUS 32）[ S ].
[ 8 ] Bahgat Eman A, Hashem Hisham, Saleh Hanaa, et al.HPLC-DAD technique for the quantification of a recently approved anti-diabetic triple combination along with two toxic official impurities： Toxicity confirmation aided by molecular docking application[ J ]. BMC Chemistry, 2023, 17（ 1 ）：18.
[ 9 ] Souza Paulo Victor R, Martins Vanessa G F C, Chagas Maria do S-
ocorro S, et al, Validation of a New HPLC-DAD Method to Quantify 3-Deoxyanthocyanidins Based on Carajurin in Medicinal Plants and for Chemical Ecology Studies[ J ]. Plants, 2023, 12（ 5 ）：1 057.
