Studies on HPLC method of R-3-aminobutyric acid related substances in aspartase system
Mu Yumin4, Han Guangxin1, Zhang Haoyue4, Liang Hailong4, Yang Mengxi4, Liu Lirong4, Ren Limei1，2，3
1. School of Chemical Engineering, Shijiazhuang University, Shijiazhuang 050035, China; 2. Hebei International Joint Research Center for Biopharmaceuticals, Shijiazhuang 050035, China; 3. Hebei Provincial University Microbial Pharmaceutical Application Technology R&D Center, Shijiazhuang 050035, China; 4. Mei bang mei he Biological Technology Corporation Ltd., Shijiazhuang 050000, China
Abstract： In order to establish an efficient analysis method of R-3-aminobutyric acid and related substances in the enzymatic system. Based on the color development method, the derivative method, the method of HPLC external standard at 210nm with specific mobile phase and detection parameters was explored. The results show that under selected chromatographic conditions, R-3-aminobutyric acid and related substances can be well separated from other substances in the enzymatic reaction, and the substrate crotonic acid in the concentration of 0.05 ~ 0.4 g/L Range lineargood, R2More than 999, the product R-3-aminobutyric acid has good sensitivity under this detection conditions, the detection limit and quantification limit meet the requirements, and the concentration is 0.35 ~ 2 g/L Range lineargood, R2More than 999. The method also meets the raw materials, catalytic reaction purification and crystallization, finished product content and impurity detection.
穆玉敏4，韩广欣1，张浩月4，梁海龙4，杨梦茜4，刘丽荣4，任丽梅1, 2, 3. 天冬氨酸酶体系中R-3-氨基丁酸相关物质HPLC方法研究[J]. 煤炭与化工, 2023, 46(5): 122-125，129..
Mu Yumin4, Han Guangxin1, Zhang Haoyue4, Liang Hailong4, Yang Mengxi4, Liu Lirong4, Ren Limei1，2，3. Studies on HPLC method of R-3-aminobutyric acid related substances in aspartase system. CCI, 2023, 46(5): 122-125，129..
