高锰酸钾氧化脱色技术在肝素制备中的应用研究

doi:10.19286/j.cnki.cci.2025.07.029

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摘要

探究了肝素钠提取纯化中高锰酸钾氧化较优技术参数。选取高锰酸钾加量、氧化温度、pH值、氧化时间进行单因素实验，进一步通过正交实验对肝素的高锰酸钾脱色条件进行优化。考察了氧化后肝素的脱色率、抗Ⅱa因子效价、收率和重均分子量等质量指标，并对双糖结构进行分析。结果显示，高锰酸钾氧化脱色的最优条件为：高锰酸钾加量1.6%，氧化温度75 ℃，pH值9.0，氧化时间4 h。双糖结构分析表明高锰酸钾氧化脱色制备的肝素ΔGlyser含量较低，产品质量更加稳定。

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	胡立娜1
	3
	张惠1
	3
	赵一凡1
	许忠良2
	郄正刚1
	李志敏1
	3

关键词 ：肝素, 高锰酸钾, 氧化, 脱色率, 效价, 重均分子量

Abstract：

The optimal technical parameters for potassium permanganate oxidation in the extraction and purification of heparin sodium were studied. The amount of potassium permanganate, oxidation temperature, pH vaule and oxidation time were selected for single-factor experiments. Furthermore, orthogonal experiments were used to optimize the decolorization conditions of heparin with potassium permanganate. The quality indicators for oxidized heparin such as decolorization rate, anti-factor IIa activity, yield and weight average molecular weight were investigated, and the structure of disaccharides was analyzed. Results show that the optimal conditions for the oxidation decolorization with potassium permanganate were as follows: the amount of added potassium permanganate was 1.6%, the oxidation temperature was 75 ℃, pH vaule was 9.0, the oxidation time was 4 h. The structure analysis of disaccharides showed that the content of ΔGlyser prepared by oxidation decolorization with potassium permanganate was lower, and the product quality was more stable.

Key words： heparin potassium permanganate oxidation decolorization rate potency weight average molecular weight

基金资助:

河北省多糖类药物技术创新中心绩效补助经费（No.22567688H）；石家庄市多糖类药物技术创新中心绩效后补助经费
（No.237790679A）；石家庄市中试熟化平台项目（No.234200054A）

通讯作者: 李志敏（ 1980— ），女，河北石家庄人，正高级工程师。

作者简介: 胡立娜（ 1982— ），女，河北石家庄人，工程师。

引用本文:

胡立娜1,3，张惠1,3，赵一凡1，许忠良2，郄正刚1，李志敏1,3. 高锰酸钾氧化脱色技术在肝素制备中的应用研究[J]. 煤炭与化工, 2025, 48(7): 135-140. Hu Lina1,3, Zhang Hui1,3, Zhao Yifan1, Xu Zhongliang2, Qie Zhenggang1, Li Zhimin1,3. Study on the application of potassium permanganate decolorization technology in the preparation of heparin. CCI, 2025, 48(7): 135-140.

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http://www.mtyhg.com.cn/CN/10.19286/j.cnki.cci.2025.07.029 或 http://www.mtyhg.com.cn/CN/Y2025/V48/I7/135

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