According to the distribution of micro-seismic monitoring events in the process of water inrush change in large mining depth mine, the response of groundwater inrush to floor rock strata was indirectly observed. Through the characteristics of micro-seismic events in different horizons and the law of space-time development, the role of micro-seismic technology in the dynamic monitoring of deep anomalies was studied and used as a basis to guide mining. The prevention and control of floor water damage and the development of water control works after the occurrence of water inrush were carried out.