The detection of the content of thiol groups is very important in the biological and biomedical fields. The content of the thiol group in the living body was detected using Luo Danming as a fluorescent probe containing the Se-N bond. The detection conditions: the temperature was 25℃, 0.5 mol / L rhodamine Se-N was mixed with glutathione （GSH） in pH=7.4 phosphate buffer solution（PBS）, the temperature of the solutions kept for 30 min, the fluorescence properties of the solution was detected by fluorescence analyzer, the concentrations of glutathione was detected through the changes of the spectra of the rhodamine fluorescence. The experiment results show that the Luo Danming fluorescent probe with Se-N bond has high sensitivity and selectivity in the detection of cell HL-7702 and HepG2, can be used to detect the thiol content in biological cells.
[ 1 ] 陆金鑫，马会民.检测巯基物质的光学探针研究进展[ J ]. 科学通报，2012（16 ）：1 462-1 471。
[ 2 ] 尹伶灵，陈蓁蓁. 巯醇类荧光探针研究进展[ J ]. 分析化学，2009（7 ）：1 073-1 081.
[ 3 ] 马会民，梁树权. 光学分析试剂[ J ]. 化学通报，1999（10 ）：29-33.
[ 4 ] Chen X Q, Zhou Y, Peng X J, et al. Fluorescent and colorimetric probes for detection of thiols[ J ]. Chem Soc Rev，2010，39：2 120-2 135.
[ 5 ] Chen W, Zhao, Y, Seefeldt T, et al. Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid [ J ]. J Pharm Biomed Anal，2008，48：1 375-1 380.
[ 6 ] Safavi A, Maleki N, Farjami E, et al. Simultaneous electrochemical determination of glutathione and glutathione disulfide at a nanoscale copper hydroxide composite carbon ionic liquid electrode[ J ]. Anal Chem， 2009，81：7 538-7 543.
[ 7 ] Yan B Z, Stoner D L, Scott J R. Direct LD-FTMS detection of miner-al-associated PAHs and their influence on the detection of co-existing amino acids[ J ]. Talanta，2007，72：634-641.
