At present, because most fluorescent probes of Cu2+ have the shortcomings as low sensitivity, poor selectivity, long response time, low quantum yield in aqueous solution and high cytotoxicity etc., so that biological applications of Cu2+ fluorescent probes have been greatly restricted. By grafting 4-aminoantipyrene on the coumarins skeleton, a novel fluorescent probe L was synthesized; through UV-visible spectra and fluorescence spectra, the interaction and action mechanism of Cu2+ fluorescent probes L and Cu2+ were studied, interference of other metallic ion on probe L was studied. The result shows that probe L has a high selectivity and sensitivity on Cu2+.
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