Phosphatidic acid(PA) is the basic composition of cell membrane, nuclear membrane and plasmid membrane of the animal and plant, and also is the foundation of life. PA can be used in food, medicine and cosmetics. PA’s nature and structure was introduced and its preparation methods and detection methods were introduced in detail. PA’s preparation methods include the thin layer chromatography (TLC), solvent extraction, enzyme modification, chemical synthesis. Meanwhile, PA’s detection methods include thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). It is prospected that the PA’s research devevopment tendency will focus on the industrialized preparation technology,so its application scope can be enlarged.
陶银华，李京东，刘 玮，汤善海. 磷脂酸制备研究进展[J]. 煤炭与化工, 2015, 38(2): 38-39,113.
TAO Yin-hua, LI Jing-dong, LIU Wei，TANG Shan-hai. Research Progress on Preparation of Phosphatidic acid. CCI, 2015, 38(2): 38-39,113.
[ 1 ] Medh J D, Weigel P H. Separation of phosophatidylinositols and other phospholipids by two-step one-dimensional thin-layer chromatography[J]. Lipid Res,1989,30:761-764.
[ 2 ] Nishide T, Yasumura D, Nakajima Y,et al. Processes for the production of phospohatidic acid[ P ]. US:5 183750,1993.
[ 3 ] 唐亚楠，徐 璐，郑建仙.苦味抑制剂磷脂酸的制备工艺优化 研究[J].食品与机械,2010,4:102-104.
[ 4 ] Chen F M , Chen M G, Sun D W. Methods for preparing phosphatidic acid or salt of phosophatidic acid [P]. CN： 1075505C,2001.
[ 5 ] Albert J. Dijkstra, Jan De Kock, Denderleeuw. Process for fractionating phosphatide mixtures[P]:US 5214171 1993.
[ 6 ] 陶银华. 磷脂酸的提取纯化及生理活性研究[D].山东师范大学,2008.
[ 7 ] Angelo St, Allen J, Jmaes, C Jr. Analysis of lipids from cooked beef by thin-layer chromatography with flame-ionization detection[J].J AO C S,70(12):1 245-1 250.
[ 8 ] 夏海涛, 刘玉芬，侯进龙. 大豆磷脂中磷脂酞胆碱的脂肪酸组成分析[J].分析化学, 2003, 31(l):126.
[ 9 ] M AWATARI S , M URAKAMI K.Analysis of membrane pho spholipid per oxida tion by isocratic high-performance liquid chromatog raphy with ultr avio let detection [J] .Analytical Biochemistry,1998,264:118-123.
[10] Rhee J S，Shin M G. Analysis of Phosphatidylcholine in SoyLecithins by HPLC[J]. J A O C S, 1982,59(2):98-99.
[11] Row K H， Kang D H. Nomral-Phase high-Perfromance liquid chromatography of Phospholipids from soybean with evaporative light scattering detection[J]. American Biotechnology Laboartory,2003(8):40-42.
[12] Meeren P Van Der, Vanderdeelen J, Huys M,et al. Simple and rapid method for high-performance liquid chromatographic separation and quantification of soybean phospholipids[J].Chormatogr,1988,447:436-442.
[13] Amari J V, Brown P R. Isolation and purification of lecithin by preparative high-performance liquid chromatography[J].Chromatogr,1990, 517:219-228.
[14] 何新霞，郑孝华，郏海燕,等.HPLC法测定大豆磷脂中卵磷脂含量[J].食品科学,2000, 21(2):57-59.
[15] Caerlli A A, V Brevedan M I, Crpaiste G H. Quantitative Determination of Phospholipids in Sunflower oil[J].J A O C S,1997,74(5):511-514.
[16] 杨 玲, 何新霞. HPLC分析大豆磷脂胶囊的磷脂组分[J].食品科学.2000,21(2):53-54.
