Determination of fecal coliform group in water——comparison of enzyme substrate method and fluorescence spectrophotometry
Zhang Yin1，Liu Xinan2，Wang Jing3，Liu Juan3
1. Hebei Province Ecology Environmental Monitoring Center, Shijiazhuang 050035, China; 2. China South to North Water Diversion Group Middle Route Corporartion Ltd., Hebei Brancht, Shijiazhuang 050035, China; 3. Shijiazhuang Hi-tech Industrial Development Zone Water Supply and Drainage company, Shijiazhuang 050035, China
Abstract： Enzyme substrate method and fluorescence spectrophotometry were used to detect fecal coliform group in source water, landscape water and sewage. Compared with the method of enzyme substrate and fluorophotometry, the result of fluorophotometry is less relative error, closer to the true value and more accurate. The relative standard deviation of fluorometric method was lower than that of enzyme substrate method, indicating that the stability of fluorometric method was better than that of enzyme substrate method. Two methods were used to determine different types of samples, and the results were statistically analyzed. It was found that the two methods had correlation in statistics, and there was no significant difference. The fluorometric method can be used to determine fecal coliform group in a short time, reduce the human error and avoid the influence of the measuring time on the results, especially suitable for emergency detection.
