Denatonium saccharide is one of the two bitterest substances known in the world. By adding it to methanol, industrial oils and toxic household and vehicular agricultural chemicals that are easily ingested, it gives them a bitter taste and prevents people from ingesting it.Denatonium saccharide was prepared from denatonium benzoate aqueous solution and saccharin sodium with distilled water through one-step post-treatment. This method has the advantages of saving a lot of solvent, reducing the production cost, simplifying the synthesis process, reduc the discharge of waste liquid, and is more conducive to industrial production and improving production efficiency.