Application research of hydrophobic chromatography in the separation and purification of recombinant human serum albumin
Cui Bin1,2, Zhang Rong1
1. College of Life Sciences and Biopharmaceuticals, Shenyang Pharmaceutical University, Shenyang 110016, China；
2. Biotechnology Branch, North China Pharmaceutical Corporation Ltd., Shijiazhuang 052165, China
Recombinant human serum albumin （rHSA）has being used in the production of high-dose biological products, which poses a great challenge to the downstream separation and purification technology. Hydrophobic chromatography separation is achieved by using the interaction between hydrophobic groups of sample components and hydrophobic groups of media, which can effectively remove degradants in the purification of rHSA. Phenyl Sepharose 6FF (HS) hydrophobic separation medium from GE Company was selected to separate and purify rHSA intermediates, and the process control conditions were optimized. The experimental results show that the best removal efficiency and protein yield are obtained by using 50 mmol/L PB + 0.1 mol/L NaCl buffer and controlling pH value of 6.0.
崔 斌1,2，张 嵘1. 疏水色谱分离在重组人血白蛋白分离纯化中的应用研究[J]. 煤炭与化工, 2019, 42(3): 133-136.
Cui Bin1,2, Zhang Rong1. Application research of hydrophobic chromatography in the separation and purification of recombinant human serum albumin. CCI, 2019, 42(3): 133-136.
