BOD₅ measuring method and process of landfill leachate were discussed.With the city sewage treatment plant biological tank effluent as inoculum source, inoculated liquid was cultivated and acclimated  by SBR operational model. The BOD₅ of the  inoculated dilution was 0.50 mg/L, which was conforming the requirement. For high concentrations of organic matter, the BOD₅ of leachate was measured with 2 000, 2 500 and 3 000 three dilution multiples. And each dilution multiple was made two parallel samples. The results showed that the consumption and surplus of DO were more than 2 mg/L, which were conforming the requirement. The averaging value of BOD₅ was 8.58 × 10³ mg/L.