辅酶A测定方法综述

doi:10.19286/j.cnki.cci.2022.06.038

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摘要辅酶A (CoA)是普遍存在的细胞分子，能介导数百种合成代谢和分解代谢反应，包括能量代谢。CoA通常用于白细胞减少、特发性血小板减少性紫癜和功能性低温症的辅助治疗。建立了一种灵敏、特异的分析方法来监测不同生物基质中CoA的浓度，综述了近三十多年测定CoA的多种方法，列出了每种方法的检测原理、检测模型、检出限、检测范围等分析参数，并评价了各种方法的优缺点。研究有助于增强辅酶A定量方法论在生物基质中的识别与定量，并加深对其在人类健康和疾病中所起作用的理解。

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	刘里
	石柔

关键词 ：辅酶A, 测定和综述, 荧光分光光度法

Abstract： Coenzyme A (CoA) is a ubiquitous cellular molecule that can mediate hundreds of anabolic and catabolic reactions, including energy metabolism. CoA is usually used as an adjuvant treatment for leukopenia, idiopathic thrombocytopenic purpura and functional hypothermia. A sensitive and specific analytical method was established to monitor the concentration of CoA in different biological substrates, various methods for the determination of CoA in recent 30 years were reviewed, the detection principle, detection model, detection limit, detection range and other analysis parameters of each method were listed, and the advantages and disadvantages of each method were evaluated. The research will help to enhance the identification and quantification of CoA quantitative methodology in biological matrix, and deepen the understanding of its role in human health and disease.

Key words： coenzyme A determination and review fluorescence spectrophotometry

基金资助:国家自然科学基金资助项目（21804079）；云南省地方本科高校（部分）基础研究联合专项资金重点项目（202001BA07000 1-127）；上海师范大学资源化学教育部重点实验室开放课题；2021年云南省大学生创业训练项目（s202110684026x）

作者简介: 刘里（ 1982— ），女，吉林吉林人，副教授。

引用本文:

刘里，石柔. 辅酶A测定方法综述[J]. 煤炭与化工, 2022, 45(6): 140-147，157.. Liu Li， Shi Rou. Review on the determination method of coenzyme A. CCI, 2022, 45(6): 140-147，157..

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