The continuous coordination titration experiment in analytical chemistry experiment is the continuous determination of lead and bismuth content. As an important traditional experiment of coordination titration method, it is widely carried out in undergraduate experimental teaching of colleges and universities. However, the high toxic reagents such as bismuth nitrate and lead nitrate used in the experiment have outstanding problems such as high operational risk and difficult treatment of waste liquid. From the perspective of green chemistry, the determination object from Bi3+-Pb2+ to Fe3+-Zn2+ was changed the continuous determination of metal ions in a single test solution was and realized, the requirements of experimental teaching objectives were met . The precision and accuracy verification showed that the method had no significant system error and met the quantitative requirements of analytical chemistry. The improved experimental system deeply integrates the concept of green environmental protection teaching, improves the experimental safety from the source, reduces the toxicity of waste liquid, and significantly reduces the experimental cost. The green experiment improvement has both teaching applicability and technical feasibility, which provides an innovative model for the sustainable development of analytical chemistry experiment courses in colleges and universities, and has promotion value.