Avermectin,which has the antiparasitic activity, is a secondary metabolite produced by srteptomyces avermitilis. The improvement of the strain is the key factor to raise theaverrmectin production. By inducing the avermectin protoplast and L-Ile being as a select model, the high production strain fistly was selected, the avermectin protoplast was prepared. The protoplasts were induced by UV radiation at the condition of stiring, lastly they were cultured on the plate containing 0.5% L-Ile. A new strain was obtained which potency of B1a has raised 35.4% than the beginning strain.
[ 1 ] Burg R W, Miller B M, Baker E E, et al.Avermectins,new family of potent anthelmintic agents:Producing or-ganism and fermentation[ J ].Antimicrob Agents Che-mother，1979，15（3）：361 - 367.[ 2 ] 贺筱蓉，黄小倩链霉菌原生质体的制备[ J ]. 生物学通报，1988，33（1）：40.[ 3 ] 王晓伟，何建勇，白秀峰. 阿维菌素E107异常代谢产物的研究[ J ]. 中国抗生素杂志，2004，21（3）：222 - 225.[ 4 ] Hopwood D A, Bibb M J, Chater K F, et al.GenetiManipulation of Streptomyces. A Laboratory Manua[ M ]. England: John Innes Foundation Press, 1985：84 - 170.[ 5 ] 张 毅. 阿维菌素高产菌株的选育[ D ] . 武汉：华中农业大学植物科技学院，2008.[ 6 ] 朱林东，金志华. 普那霉素产生菌的原生质体诱变育种[ J ]. 中国抗生素杂志，2006，31（10）：591 - 594.[ 7 ] 梁剑光，储消和，储 炬，等. 阿维菌素高产工业菌株的推理选育研究[ J ]. 安徽农业科学，2011，39（24）：14 660-14 662，14 683.[ 8 ] 张红梅. 漆酶产生菌的原生质体诱变选育[ J ]. 郑州轻工业学院学报，2005，02.
