In order to optimize the pretreatment method of anaerobic digestion technology, and then seek environmental protection, efficient treatment way of crop straw, the decomposition process of cellulose under natural conditions was studied, using the collaborative relationship between microbes, according to its acid-base complementary principle, cellulose degrading bacteria with different acid-base reactions ware composed and optimized, thus, a stable and efficient cellulose degrading compound agent was obtained by screening and domestication. The results showed that the bacterial agent was applied to the biological treatment of corn straw, the methane yield of corn straw pretreated for 15 days and 20 days was (131 ~ 239)mLNCH4/gTS, which was 26.70% ~ 75.57% higher than that of untreated corn straw, and the biodegradability and bioenergy yield of corn straw were effectively increased.
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