etermination of Vitam C in Kiwifruit by High Performance Liquid Chromatography
LI Hui-jing1，ZHANG Jian-li1，WANG liang2
1. Hebei Welcome Pharmaceutical Corporation Ltd.,Shijiazhuang 050031, China; 2. Aino Corporation Ltd., North China Pharmaceutical Group, Shijiazhuang 052165, China
A method of detecting the content of vitamin C in the kiwifruit by HPLC was established. A HYPERSI L - C18 column was used,the flow phase was 0.1% oxalicacid, the flow rate was 1.0 mL/min and the detection wavelength was 254 nm in the HPLC system. The standard working curve of vitamin C had a good linear relation in the concentration range of 10～500μg／mL, the linear equation was y =125.51x +225.54 and the related coefficient was 0.998 7,the recoveries were 79.2%~98.7%. This method was simple,fast and suitable to detect the content of vitamin C in kiwifruit.
李会静，张建利，王 亮. 高效液相色谱法测定猕猴桃中维生素C的含量[J]. 煤炭与化工, 2013, 36(4): 140-141.
LI Hui-jing，ZHANG Jian-li，WANG liang. etermination of Vitam C in Kiwifruit by High Performance Liquid Chromatography. CCI, 2013, 36(4): 140-141.
