Rock burst accidents in coal mines frequently occurred in high in-situ stress areas of hard and brittle coal-rock masses and coal seams with a burial depth exceeding 400 m. With severe hazards, such accidents were caused, bringing huge economic losses and casualties to coal mines. The mining depth of No. 3 coal seam in Changping Coal Industry was more than 400 m, which met the critical mining depth standard for rock burst-prone coal mines in China. To prevent the occurrence of rock burst accidents in No. 3 coal seam, rock burst tendency tests and risk assessment analyses were carried out in this study. The test results were presented as follows: the average values of dynamic failure time, elastic energy index, burst energy index and uniaxial compressive strength of No. 3 coal seam in Changping Coal Industry were determined to be 2 312 ms, 3.06, 1.94 and 6.55 MPa, respectively. Through comprehensive judgment, No. 3 coal seam in Changping Coal Industry was identified as non-rock burst tendency. The bending energy indexes of roof and floor strata of No. 3 coal seam were calculated as 11.867 kJ and 7.174 kJ respectively, both of which were less than 15 kJ, and the roof and floor strata were classified as non-rock burst tendency. In accordance with the comprehensive index method of coal seam geological factors and mining technical factors, the rock burst risk level of No. 3 coal seam in Changping Coal Industry was evaluated as non-rock burst risk. The relevant test data were provided in this research, which can supply basic parameters and decision-making bases for mine mining and rock burst prevention and control, and the safe and efficient production of the mine was guaranteed.