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| Study on HPLC analysis method of glutathione in multienzyme system |
| Zhang Haoyue1， Mu Yumin1， Qin Haojie1， Yang Mengxi1， Liu Lirong1， Ren Limei2， 3， 4 |
| 1. Meibangmeihe Biological Technology Corporation Ltd.， Shijiazhuang 050000， China1; 2. Shijiazhuang University， School of Chemical Engineering， Shijiazhuang 050035， China; 3. Hebei International Joint Research Center for Biopharmaceutical， Shijiazhuang 050035， China; 4. Hebei Provincial University Microbial Pharmaceutical Application Technology R and D Center， Shijiazhuang 050035， China |
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Abstract In order to establish a rapid and effective method for the qualitative and quantitative determination of complex components of glutathione produced by enzyme catalysis in vitro. According to the properties and characteristics of each component of the reaction liquid， a new separation method was established by HPLC. The results showed that the separation degree of L-cysteine， L-sodium glutamate， ATP， AMP， ADP， reduced glutathione and oxidized glutathione was greater than 1.5， and the separation degree of L-cysteine and glycine was greater than 1.2; The linear R2 of the substrate and product curves was greater than 0.99， the recoveries were between 94.6% and 110.0%， and the RSDS were all less than 2.0% for 6 consecutive tests; Moreover， the detection time of a single sample is 15 min， compared with the pharmacopeia tablet detection method of 45 min， the detection time is greatly shortened， the test results can be quickly issued， and it is more conducive to daily analysis and detection.
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