Determination of penicillin G drug in pharmaceutical mushroom residue by liquid chromatography - tandem mass spectrometry
Shi Qi1, Zhong Weizhang1, Meng Wenru1, Zhang Tao1, Wang Yongjun2, Wang Honghua3, LI Zaixing1
1. School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China; 2. NCPC Environmental Protection Research Corporation Ltd., Shijiazhuang 050015, China; 3. Hebei Province Environmental Sciences Institute, Shijiazhuang 050051, China
The determination method of penicillin G residue amount in penicillin mushroom residue by liquid chromatography-tandem mass spectrometry ( LC-MS/MS ) was established. First 20% zinc acetate and 10% potassium ferrocyanide solution was used as protein precipitant for pretreating the samples. Then 0.1% formic acid aqueous solution and acetonitrile was used as mobile phase for gradient elution. Finally the Plus C18 column was used as the separation column for MRM detection with tandem mass spectrometry ESI ( + ) mode. The method detection limit of penicillin G ( S / N = 3 ) is 1.4 ng/mL, the linear range is 1.4～150 ng/mL, the linear correlation coefficient is 0.999 5, the sample detection concentration and the limit of quantification are 1.8 g/kg and 3 g/kg respectively. The recovery rate of each component is all 76.3% ～93.2% at three levels of 9.0、40 and 80 g/kg, and the relative standard deviation is <5%. The method is accurate for qualitative and quantitative analysis, and has good reproducibility, and can satisfy the requirements of detecting penicillin G residue in pharmaceutical mushroom residue.
师 奇1，钟为章1，孟文茹1，张 涛1，王勇军2，王洪华3，李再兴1. 菌渣中青霉素G的液相色谱-串联质谱测定法[J]. 煤炭与化工, 2018, 41(3): 16-19.
Shi Qi1, Zhong Weizhang1, Meng Wenru1, Zhang Tao1, Wang Yongjun2, Wang Honghua3, LI Zaixing1. Determination of penicillin G drug in pharmaceutical mushroom residue by liquid chromatography - tandem mass spectrometry. CCI, 2018, 41(3): 16-19.
