In the BrO₃--Mn²⁺-H⁺-CH₃COCH₃ oscillation system，radix bupleuri was used as reaction substrate to get the electrochemical fingerprint of radix bupleuri through electrochemical workstation. In single factor experiment, the desired electrochemical fingerprints could be achieved when the speed was 760 r/min, the quantity of radix bupleuri was 0.8g, the temperature was 40 ℃, the particle size was 40 mesh. Under the condition of the determination, the electrochemical fingerprints of radix bupleuri from different areas were analyzed. The characteristics of electrochemical fingerprint physical parameter were adopted in order to identify authenticity of radix bupleuri and evaluate the quality of radix bupleuri in different regions.