In order to improve the speed of cell producing sorbose and short the fermentation period, the effects of  the main organic acids in tricarboxylic acid cycle on the fermentation of sorbitol were researched.The single factor screening experiment showed that malic acid, CIS aconitic acid, oxaloacetic acid and succinic acid could promot sorbitol fermentation, and fumaric acid had no obvious effect on sorbitol fermentation. The levels and factors of orthogonal experiment were determined according to the single factor experiments, the optimum compounding of organic acids was malic acid 0.06%, CIS aconitic acid 0.015%，oxaloacetic acid 0.10%, succinic acid 0.04% through orthogonal experiment.The sorbitol fermentation period was shortened by 6 hours through small fermentor pilot-scale experiment.