肽链的N，C末端对抗菌肽活性的影响研究

doi:10.19286/j.cnki.cci.2018.03.037

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Abstract

In order to study the effect of N and C terminal of peptide chain on the activity of antimicrobial peptides, two antimicrobial peptides were designed and synthesized. One is the antimicrobial peptide with melittin Anoplin as N terminal and Mastoparan (MP)as C terminal.GI-1 made up of 25 amino acid residues connected Pro. The other is IG-1 melittin Anoplin as C terminal with and the mastoparan (MP) IG-1 as N terminal. The experimental results show that the antitumor activity of two antimicrobial peptides in vitro is not significantly different, and IG-1 is slightly superior to GI-1. Both peptides are less lethal to normal cells (NIH-3T3) than to tumor cells (HEPG-2), indicating that they have certain selective toxicity. In addition,the hemolytic activity of IG-1 is significantly higher than that of GI-1.

Key words： N,C terminal inhibition ratio hemolytic activity selective toxicity

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	Articles by authors
	Liu Liwei
	Cui Fuxuan
	Han Xiaoyan

Cite this article:

Liu Liwei,Cui Fuxuan,Han Xiaoyan. Effects of peptide N and C terminal on the activity of antimicrobial peptide[J]. CCI, 2018, 41(12): 120-122.

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http://www.mtyhg.com.cn/EN/10.19286/j.cnki.cci.2018.03.037 OR http://www.mtyhg.com.cn/EN/Y2018/V41/I12/120

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