Research of in-situ stress is of great importance for rock control and dynamic disaster prevention in coal mine. Based on analysis of evolution characteristics of stress field and in-situ stress measurement, the type and magnitude of stress field were learned and effect of fold structure on in-situ stress was studied. By the inversion method of multiple linear regression analysis, the in-situ stress field distribution of mining area of fold stereotype was studied. Furthermore, the relation of in-situ stress and disasters in fold stereotype mining area was analyzed, and the application methods of in-situ stress measurement and inversion on design of rock stability and dynamic disaster prevention were studied. The research showed as following, firstly, affected by the geological structure significantly, the type of in-situ stress field was horizontal-master stereotype, which was σH> σh > σv. Secondly, the stress value of fold axis was much bigger than the other zones, and the maximum principal stress direction was vertical with the axis’ trend, and however the one of wing will deflect. Thirdly, according to the inversion results of in-situ stress, the greater folds varied, the bigger in-situ stress value become. Lastly, on account of the closely link of roadway stability, impact, coal and gas outburst and in-situ stress, the mine roadway optimization and disaster prevention can be guided effectively through the in-situ stress measurement and inversion.