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| Determination of penicillin G drug in pharmaceutical mushroom residue by liquid chromatography - tandem mass spectrometry |
| Shi Qi1, Zhong Weizhang1, Meng Wenru1, Zhang Tao1, Wang Yongjun2, Wang Honghua3, LI Zaixing1 |
| 1. School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China; 2. NCPC Environmental Protection Research Corporation Ltd., Shijiazhuang 050015, China; 3. Hebei Province Environmental Sciences Institute, Shijiazhuang 050051, China |
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Abstract The determination method of penicillin G residue amount in penicillin mushroom residue by liquid chromatography-tandem mass spectrometry ( LC-MS/MS ) was established. First 20% zinc acetate and 10% potassium ferrocyanide solution was used as protein precipitant for pretreating the samples. Then 0.1% formic acid aqueous solution and acetonitrile was used as mobile phase for gradient elution. Finally the Plus C18 column was used as the separation column for MRM detection with tandem mass spectrometry ESI ( + ) mode. The method detection limit of penicillin G ( S / N = 3 ) is 1.4 ng/mL, the linear range is 1.4～150 ng/mL, the linear correlation coefficient is 0.999 5, the sample detection concentration and the limit of quantification are 1.8 g/kg and 3 g/kg respectively. The recovery rate of each component is all 76.3% ～93.2% at three levels of 9.0、40 and 80 g/kg, and the relative standard deviation is <5%. The method is accurate for qualitative and quantitative analysis, and has good reproducibility, and can satisfy the requirements of detecting penicillin G residue in pharmaceutical mushroom residue.
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| [ 1 ] 郑庆飞. 抗生素与人类生活[ J ]. 百科知识，2016（ 6 ）：35 - 37.
[ 2 ] 李再兴，田宝阔，左剑恶，等. 抗生素菌渣处理处置技术进展[ J ]. 环境工程，2012，30（ 2 ）：72 - 75.
[ 3 ] 孙绍锋，郝永利，许 涓，等. 解析《 国家危险废物名录 》[ J ]. 中国环境管理，2013，5（ 2 ）：46 - 48.
[ 4 ] 程慧娟. 动物性食品中青霉素残留的研究进展[ J ]. 中国公共卫生，2000，16（ 7 ）：655 - 655.
[ 5 ] 田国宝，王红宁，张安云，等. 规模化猪场大肠杆菌β-内酰胺类药物耐药性检测及其超广谱β-内酰胺酶调查[ J ]. 中国预防兽医学报，2011，33（ 10 ）：776 - 780.
[ 6 ] 夏利宁，赵红琼，苏 艳，等. 新疆某猪场分离的大肠杆菌对抗生素耐药性调查[ J ]. 新疆农业科学，2014，49（ 1 ）：2 299 - 2 303.
[ 7 ] 陈 云，李洪亮，张冬洁，等. 乳中抗生素残留及其检测方法的研究进展[ J ]. 中国乳品工业，2012，40（ 9 ）：35 - 38.
[ 8 ] 李欣南，韩镌竹，苏崴艺. 超高效液相色谱-串联质谱法同时测定生鲜乳中7种β-内酰胺类抗生素残留[ J ]. 现代畜牧兽医， 2013（ 11 ）：40 - 46.
[ 9 ] 郎 友，单 艺，满朝新，等. 超高效液相色谱-串联质谱法测定乳及乳制品中青霉素类药物残留量[ J ]. 中国乳品工业，2013，41（ 5 ）：48 - 51.
[ 10 ] 李再兴，张非非，左剑恶，等. SPE-UFLC-MS/MS法测定制药废水中四环素类抗生素[ J ]. 环境科学与技术，2013，36（ 7 ）：139 - 142.
[ 11 ] 孙奉翠. 土壤中四类典型抗生素的同时测定及其方法优化[ D ]. 山东大学，2013.
[ 12 ] 付体鹏，李 莉 ，张 峰， 等.高效液相色谱-串联质谱法测定 动物肺组织中青霉素G和青霉素V[ J ]. 安徽农业科学， 2013（ 2 ）：638 - 639.
[ 13 ] 刘慧娟，王丽丽，邓凯顺. 仪宏青霉素酶在青霉素菌渣无害化处理中的应用[ J ]. 生物加工过程，2009，7（ 5 ）：54 - 57.
[ 14 ] 张兆起. 沈庆英高效液相色谱法测定青霉素G发酵液中菌体浓度[ J ]. 中国抗生素杂志，1999（ 2 ）：142 - 142. |
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