组合生物学在药物研究中的应用进展

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Abstract

Natural products with structural diversity and broad biological activities are the secondary metabolites of host cells, in contrast, the main sources of new drugs are chemical synthesis analogues. Since genetically modified heterologous hosts can efficiently express the pathway of combinatorial biosynthesis, increase the concentration of the products and reduce the costs, it is a environmental friendly way to obtain natural product analogues. Three strategies of combinatorial biosynthesis are discussed, they are: precursor-directed biosynthesis; enzyme-level modification, including the exchange of function domains, modules and subunits, site-specific mutagenesis and directed evolution; pathway-level recombination. Combinatorial biosynthesis using the broadness of substrate, enzyme engineering and catalyzing way to obtain new unnatural natural products and the structure of potential compounds become more diverse. High-throughput synergy screening methods are needed urgently to screen large amount of candidate compounds, the computational methods and structure-bioactivity analyses are combined together to conduct optimal design and then to ensure the desired activities are achieved. And the dynamics, mechanisms and the metabolic pathways of enzyme are further discussed.

Key words： combinatorial biosynthesis natural product polyketide synthase nonribosomal peptide synthetases

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	Articles by authors
	LI Yan-mei
	WANG Ya-li
	BAN Lu

Cite this article:

LI Yan-mei,WANG Ya-li,BAN Lu. Application Progress of Combinatorial Biology in Drug Research[J]. CCI, 2015, 38(7): 30-32.

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http://www.mtyhg.com.cn/EN/ OR http://www.mtyhg.com.cn/EN/Y2015/V38/I7/30

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