Non-final sterilized squirt needs to be verified by sterile simulation filling process,the validation process needs to follow the worst condition selection principle.According to the exceptions that may occur in the actual production  process to choose the worst conditions. It is pointed out that the main factors affecting the aseptic filling are production environment, production personnel, ampoules specifications, filling instruments for production,filling speed, volume adjustment etc.; only processing the filling simulation test under the worst conditions, qualified sterile culture production samples can be released.